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Confirmation of specific matrix metalloproteinase-25 (MMP-25) knockdown for in vitro palatal cultures. (A) Quantitative real-time PCR shows a significant decrease in MMP-25 mRNA levels from in vitro cultures following treatment with 500 nM MMP-25 siRNA (p < 0.01). Error bars indicate standard error of the mean. (B) MMP-25 protein expression is reduced in in vitro palatal cultures following treatment with MMP-25 siRNA. β-Actin was used as a loading control.
