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Matrix metalloproteinase-25 (MMP-25) mRNA and protein expression is decreased in in vitro palatal cultures following treatment with a TGF-β3-neutralizing antibody. (A) Quantitative real-time PCR data indicates a significant reduction in MMP-25 mRNA levels in in vitro palatal cultures after exposure to a TGF-β3-neutralizing antibody. (B) MMP-25 protein is decreased upon bio-neutralization of TGF-β3. β-Actin was used as a loading control.
