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Western blot analysis against phospho-Smad1 shows a TGF-β3-neutralizing antibody concentration-dependent effect. Approximately 10 μg of total protein was separated via SDS-PAGE and probed with an anti-phospho-Smad1 antibody (Cell Signaling Technology; 1:500). An anti-β-actin antibody was used to assess loading equivalence (Santa Cruz; 1:1000).
