Table 1.
Spot | Acc. number | Protein identitya | PM/Seq cov./scoreb | Best peptides (PRO-BLAST) | Identified by | Experimental MW/pI | Theoretical MW/pI | Organism | Signal P |
---|---|---|---|---|---|---|---|---|---|
S1 | gi|256724272 | Hypothetical protein | 2/20%/83 | MASCOT | 13.2/7.6 | 13.5/9.6 | Nectria haematococca | NNc | |
S2 | gi|255935271 | Pc13g02210 | 2/14%/79 | KIIYPAYTDK ARIKGDVVKPDKSYAPKAIP |
PRO-BLAST | 13.2/7.8 | 14.5/3.7 | Penicillium chrysogenum | 0.999 |
S3 | gi|256724272 | Hypothetical protein | 2/20%/82 | MASCOT | 13.4/5.6 | 13.5/9.6 | Nectria haematococca | NN | |
S4 | gi|27924024 | glucosidase | 4/11%/97 | GFNIGATNADGSC*Kd EGGHVSISAVSRSEDLYRGDSDASK |
PRO-BLAST | 30.2/4.7 | 33.4/9.3 | Fusarium sporotrichioides | 0.999 |
S5 | gi|256732553 | Predicted protein (necrosis and ethylene inducing peptide 2 precursor) | 2/7%/97 | GELHNDAFALFYAWYFAPG AVLAHDAVVGFAETVPFVGVWHAHK |
PRO-BLAST | 35.7/4.7 | 24.69/7.75 | Nectria haematococca | 0.999 |
S6 | gi|239931549 | Secreted protein | 2/9%/105 | SGASSWTAAR SSSFPHWLTLSGSC*NTR |
PRO-BLAST | 26.3/4.2 | 23.03/8.35 | Streptomyces ghanaensis | 0.989 |
S7 | gi|189192036 | Hypothetical protein | 3/18%/94 | MASCOT | 19.4/5.1 | 22.5/7.6 | Pyrenophora tritici-repentis | 0.999 | |
S8 | gi|256726602 | Hypothetical protein (necrosis-and ethylene-inducing protein 1) | 3/8%/77 | FGLFYAWYFPK ALSPHDSLTKDSYVR |
PRO-BLAST | 33.8/5.5 | 26.99/9.2 | Nectria haematococca | 1.000 |
S9 | gi|156061869 | Hypothetical protein (necrosis-and ethylene-inducing protein 1) | 6/21%/77 | MASCOT | 26.0/5.2 | 26.9/6.6 | Sclerotinia sclerotiorum | 0.994 | |
S10 | gi|189196154 | NADP-specific glutamate DH | 9/31%/302 | MASCOT | 48.0/6.9 | 49.1/5.9 | Pyrenophora tritici-repentis | No | |
S11 | gi|171677093 | Unnamed protein product (aspartate aminotransferase) | 5/19%/80 | MASCOT | 43.4/7.6 | 43.0/8.3 | Podospora anserina | No | |
S12 | gi|237868680 | Peptidase 1 | 4/8%/109 | KTSGNFIASVASMSLGFESR KC*FPTEEDRRSGVDVYVVDTGLYTAHSEF |
PRO-BLAST | 30.9/9.4 | 38.7/7.9 | Pleurotus ostreatus | 1.000 |
S13 | gi|255946596 | Pc22g00190 (cell wall protein PhiA) | 2/9%/81 | KGIGYTTGAEPTPK ASTPKKLYVDR |
PRO-BLAST | 16.2/4.4 | 18.9/5.6 | Penicillium chrysogenum | 0.996 |
S14 | gi|182412796 | Hypothetical protein | 2/2%/81 | FGHWSPNASLATAAK YGPLSFVKGK |
PRO-BLAST | 33.1/5.4 | 74.5/6.8 | Opitutus terrae | 0.990 |
S15 | gi|261355012 | Superoxide dismutase | 5/18%/111 | GHGLYHDHSLFWENLAC*NLWPK SYIPPLPYAYDAIEPAISR |
PRO-BLAST | 22.0/6.3 | 26.8/10.3 | Verticillium albo-atrum | No |
S16 | gi|189196326 | Glutathione reductase | 2/4%/90 | SFDPIIKDTITKEYER VAPHYLVLNGSGGLASGR |
PRO-BLAST | 58.1/7.3 | 55.8/6.1 | Pyrenophora tritici-repentis | No |
a The assignment of the biological or enzymatic function to the hypothetical proteins (based on BlastP analyses) is shown between parentheses and in italic characters.
b Number of peptides matched/Sequence coverage (%)/Score.
c NN: Non-classically secreted proteins.
d C*: Cys as S carbamidomethyl derivative