Figure 12.

The effect of homogenization and sonication on GFP activity (fluorescence). The fluorescence of GFP in protein mixture before and after homogenization was measured. GFP was extracted from IBs with N-lauroil sarcosine and remained in protein mixture of various proteins that compose IBs. Two aliquots of protein mixture were prepared and treated as described below: The first aliquot of the protein mixture was homogenized using high pressure homogenizer; five passages were made at the same conditions, usually used for IBs isolation from bacterial cells. The second aliquot of the protein mixture was sonicated with 40% power output at the same conditions, usually used for IBs isolation from bacterial cells.