Figure 4.
Time kinetic analysis of ZP1273-551aa induced acrosome reaction. Capacitated sperm (1 × 106/100 μl) were incubated with or without 2 μg/ml of ZP1273-551aa for varying time points and subsequently analyzed for acrosomal status by TRITC-PSA staining as described in Methods. Y-axis represents effective induction of acrosome reaction which is the percent induction of acrosome reaction in presence of ZP1273-551aa minus the percent induction of acrosome reaction observed in the presence of medium alone. Values are Mean ± SEM of 3 different experiments using semen samples from at least two different male donors. *p < 0.01, statistical significance with respect to the effective induction of acrosome reaction mediated by ZP1273-551aa at 2.5 minutes.