Fig. 1.
Diagram of the study design. Rats were divided randomly into 3 low-sodium study groups: low-sodium diet only (LS), dDAVP treatment (LSD), combination treatment with dDAVP and AT1a receptor antagonist losartan (LSDL). Rats from the 3 groups received an agar gel diet to provide 25 ml water and 15 g of nominally NaCl-free purified rodent chow with addition of 0.5 meq NaCl/day for a 4-day equilibration. For the LSD group, rats were infused with dDAVP (20 ng/h) of dDAVP for another 2 days. For LSDL rats, both dDAVP (20 ng/h) and losartan (20 mg·kg−1·day−1) were infused for another 2 days. Rats in the LS group were given vehicle infusion alone.