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Pam3CSK4 inhibits HCO3− absorption in the MTAL. MTALs from rats were isolated and perfused in vitro in control solution, and then Pam3CSK4 (1 μg/ml) was added to and removed from the bath solution. Absolute rates of HCO3− absorption (JHCO3−) were measured as described in methods. Data points are average values for single tubules. Lines connect paired measurements made in the same tubule. P value is for paired t-test. Mean values are given in results.
