Figure 1.
Success rate of detection of trypanosomes in the artificial dilution series of T. b. brucei. Cultured T. brucei brucei was diluted in cow blood at a concentration of 106 trypanosomes per millilitre and placed onto Whatman FTA cards. Lanes 1 to 3 are negative controls, lane 4 is a positive control lane 5 is a DNA marker, lanes 6 to 13 show the results of repeated PCR of a 10-7 dilution of the original stock (equivalent to 0.1 trypanosome per ml). Lanes 14 to 21 show the results of repeated PCR of a 10-6 dilution of the original stock (equivalent to 1 trypanosome per ml). Lanes 22 to 29 show the results of repeated PCR of a 10-5 dilution of the original stock (equivalent to 10 trypanosomes per ml). Lane 30 is a DNA marker.