Table 1.
Primers, sequences and PCR conditions used for the amplification of apxIA, apxIIA, apxIIIA, apxIVA, omp and apfa from Actinobacillus pleuropneumoniae
| Genes | Primer sequences (5'-3') | Annealing temperature |
Size of PCR product (bp) |
|---|---|---|---|
| apxIA | Forward: GCGGGATCCAACTCTCAGCTCGATAG | 55°C | 2520 |
| Reverse: GATGCGTCGACAGCAGATTGTGTTAAAT | |||
| apxIIA | Forward: GCGGGATCCATGTCAAAAATCACTT | 54°C | 2721 |
| Reverse: GCGAATTCAGCGGCTCTAGCTAAT | |||
| apxIIIA | Forward: ACGGGATCCTGGTCAAGCATGTTAG | 52°C | 3114 |
| Reverse: ATGCGTCGACTGCTCTAGCTAGGTTACC | |||
| apxIVA | Forward: GCCGAATTCCGCGCCTATATCTGG | 54°C | 2553 |
| Reverse: ATGCGTCGACCCCTTCGAATTGTTTC | |||
| Omp | Forward: GGAATTCACGCCTAAGGTTGATAT | 53°C | 984 |
| Reverse: GGTCGACCTTTATCTTCTTTTGTTG | |||
| Apfa | Forward: GGGCGAATTCATGCAAAAACTAAGT | 53°C | 444 |
| Reverse: TATGGTCGACTGATGCGCAGAAAT |
Note:
BamH I site: Underlined; EcoR I site: Italic; SaI I site: Bold; PCRs were run for 30 cycles.