Fig. 1.

Effect of angiotensin II (ANG II) stimulation on NF-κB expression. A–C: Western blot of CATH.a cell lysates showing the protein expression of p65 (A), IκK (B), and IκB-α (C) following stimulation with ANG II (100 nM) over the specified time course (n = 5, *P < 0.05). D and E: CATH.a cells were pretreated with losartan (Los; 1 μM) for 30 min followed by ANG II (100 nM) activation for a period of 8 h. RNA and protein were harvested, and RT-PCR and Western blotting were performed for p65 transcript (D) and protein (E). AU, arbitrary units. (n = 3, *P < 0.05.).