Fig. 5.

NF-κB-DNA binding. A: binding reactions under different treatment conditions. Lanes are as follows: 1, no nuclear extract; 2, ANG II + mutant p65 probe; 3, ANG II + p65 probe; 4, nuclear extract of cells treated with TNF-α (positive control). B: effect of DNA-protein interaction following NF-κB inhibition. Lanes are as follows: 1, ANG II + p65 probe; 2, nuclear extract of cells treated with TNF-α (positive control); 3, ANG II + p65 mutant probe; 4, ANG II + siRNA-treated cells; 5, ANG II + parthenolide-treated cells. C: time-dependent NF-κB-DNA binding over a specified time course following activation with ANG II (100 nM). (n = 5, *P < 0.05.)