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Flow cytometry analysis of Toll-like receptors (TLR) in live intact HL-1 cells. Black, unstained HL-1 cells; red, antibody-stained HL-1 cells. A: TLR-4. B: TLR-2. Horizontal lines in each trace indicate the percentage of cells staining positive for TLR. SAv-PE, streptavidin-phycoerythrin.