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. Author manuscript; available in PMC: 2010 Sep 23.
Published in final edited form as: Exp Hematol. 2009 Sep 24;37(12):1400–1410. doi: 10.1016/j.exphem.2009.09.006

Figure 2.

Figure 2

Primitive and definitive erythroid cells are differentiated from mGS–derived Flk1+ cells. mGS-derived Flk1+ cells were cultured on OP9 cells and small round cells and cobblestone-forming areas were found [(A) day 3, (D) day 9). May-Giemsa staining of a cytospin preparation [(B); day 3, (E); day 9)]. Immunostaining of E1 antigen and Ter119 (C,F). (C) E1 antigen; green. (F) E1 antigen; green Ter119; red. Nuclear staining with Hoechst 33324; blue. Magnification (A,D): ×100; (B,C,E,F): ×200.