Figure 4.

Myelolymphoid potential of mGS cells. Mac1⁺Gr1⁺, Ter119⁺, CD19⁺, and CD4⁺CD8⁺ cells were differentiated from mGS-derived Flk-1⁺ cells within OP9 culture (A,B,C) or OP9-DL1 culture (D). For Mac1⁺Gr1⁺, Ter119⁺ cells, cells were collected from 8–10 days culture (A,B). The numbers of myeloid and erythroid cells differentiated from mGS and ES cells were similar (B). For CD19⁺ (C), and CD4⁺CD8⁺ cells (D), cells were collected from 14–21 days culture. These FACS data are representative among three independent experiments.