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. Author manuscript; available in PMC: 2010 Sep 23.
Published in final edited form as: Dev Dyn. 2008 Oct;237(10):2918–2925. doi: 10.1002/dvdy.21720

Figure 3. ECs circumscribing the central space of VEGF treated allantoic spheroid exhibit properties of an endothelium.

Figure 3

A, LSCM optical section (3 μm) of a VEGF treated spheroid immunolabeled with antibodies to VE-cadherin (green) and SMαA (red). The data presented in A is representative of microscopic analysis of 4 spheroids. B and C, DIC images of a VEGF treated spheroid prior to (B) and following (C) injection of fluorescently conjugated Qdots into the central cavity. The increment of time between the image shown (B) and (C) is <1 second. Bars equal 100 μm. The data presented in B and C is representative of microscopic analysis of 6 spheroids.