TABLE 2.
Candidate Genes for Vinclozolin Action on Differentiating Testis
| Category | Gene | Expression Ratio V/C |
Cross Listed* |
GeneBank |
|---|---|---|---|---|
| Transcription | THO complex 2 (Thoc2, predicted) | 3 | E13 | AI639237 |
| Signaling | ||||
| Protein phosphatase 1, regulatory subunit 3C (Ppp1r3c) | 0.5 | A, E14 | BM390827 | |
| Guanylate cyclase 1, soluble, alpha 3 (Gucy1a3) | 0.4 | A, E14 | M57405 | |
| SH3 domain binding protein CR16 (Cr16)**/// WAS/WASL interacting protein family, member 3 (Wipf3) |
2.3 | A, E14 | U25281 | |
| Calcium/calmodulin-dependent protein kinase II, delta (Camk2d) | 1.7 | E13 | X77194 | |
| Rho guanine nucleotide exchange factor17 (Arhgef17, pred) | 0.6 | E13 | BI274338 | |
| Sushi domain containing 3 (Susd3, predicted) | 2.2 | E14 | AI407337 | |
| Receptors | Angiotensin II receptor, type 2 (Agtr2) | 2.4/0.4 | E13, E14 | BF552873 |
| Platelet derived growth factor receptor beta (Pdgfrb) | 1.9/3.9 | F, E14, E16 | BF283421 | |
| Protein Modification |
Protease, serine, 35 (Prss35) | 0.3 | A, E14 | AA866443 |
| PRP4 pre-mRNA processing factor 4 homolog B (Prpf4b, yeast) | 1.9 | E13 | BE105398 | |
| EF-hand calcium binding domain 2 (Efcab2, predicted) | 1.6 | E16 | BI299709 | |
| Immune | ||||
| Response | Thymus cell antigen 1, theta (Thy1) | 0.2 | E14 | AI145313 |
| RT1 class II, locus Bb (RT1-Bb) | 24.6 | E14 | AI715202 | |
| Growth Factors, Chemokines |
Insulin-like 3 (Insl3) | 1.9 | E16 | AF139918 |
| Prolactin receptor (Prlr) | 2 | E16 | M57668 | |
| Spondin 1 (Spon1) | 1.8 | E16 | M88469 | |
| Chemokine (C-X-C motif) receptor 4 (Cxcr4) | 1.7 | E16 | U54791 | |
| Epigenetics | Non-coding RNA expressed in the brain, repeat sequence, clone3** /// EST |
0.3 | A, E14 | BG670822 |
| SNF2 histone linker PHD RING helicase (Shprh, predicted) | 0.5 | A, E14 | BE104039 | |
| Chromogranin B (Chgb) | 0.2 | E14 | NM_012526 | |
| Non-coding RNA expressed in the brain, repeat sequence, clone 12**/// EST |
0.4 | E16 | BE099838 | |
| Enhancer of polycomb homolog 2 (Epc2, Drosophila, predicted) | 0.6 | E14 | AW918173 | |
| Similar to GTL2, imprinted maternally expressed untranslated (RGD1566401) |
3 | E13 | NM_019161 | |
| Hypermethylated in cancer 2 (Hic2, predicted) | 1.6 | E14 | BI284294 | |
| Cytotoxic granule-associated RNA binding protein 1 (Tia1) | 1.7 | E16 | BE104820 | |
| Chromodomain helicase DNA binding protein 6 (Chd6) | 2.1 | E16 | BF413591 | |
| HMT1 hnRNP methyltransferase-like 1 (S. cerevisiae)(Hrmt1l1)** /// | 0.7 | E16 | BG670963 | |
| Protein N-arginine methyltransferase 2 (Prmt2) | ||||
| Development | ||||
| Neural expressed developmentally down-regulated 4 (Nedd4) | 0.6 | E14 | BI277849 | |
| Adenomatosis polyposis coli 2 (Apc2, predicted) | 0.3 | E14 | BI294552 | |
| SRY-box containing gene 7 (Sox7, predicted) | 1.6 | E14 | AI058292 | |
| Synaptic vesicle glycoprotein 2b (Sv2b) | 2.4 | E16 | BG672437 | |
| Cytoskeleton- ECM |
Vascular cell adhesion molecule 1 (Vcam1) | 0.6 | E13,E14,C,A | NM_012889 |
| Osteoglycin (Ogn) | 0.3 | E14 | BG664221 | |
| Myosin-binding protein H (Mybphl) | 2.6 | E16 | AW252385 | |
| Cadherin 1 (Cdh1) | 2.5 | E16 | NM_031334 |
*
For any gene identified in multiple lists, the other lists in which it appears are indicated in the Cross Listed column. E13= altered by vinclozolin at E13, E14= altered by vinclozolin at E14, E16= altered by vinclozolin at E16, V= altered by vinclozolin in organ cultures, F= altered by flutamide in organ cultures, A= appeared in a list of F1 E16 vinclozolin altered transcripts published by [37] and C=appeared in a list of testis differentiation candidates published by [32]
**
gene names from Affymetrix annotation Rat230_2.na23.annot as they were referenced in List A; the rest from annotation Rat230_2.na27.annot