Fig. 1.

Both lean and obese Zucker rats underwent the following procedures. Preperfusion occurred for a period of 30 min followed by the contraction of one limb for 15 min via electrical stimulation. Metabolites were measured in muscle (gastrocnemius) removed from rats either immediately postcontraction or 25 min of recovery from contraction. To control for the effects of perfusion, the same measures were made in muscle taken from the nonexercising limb. Glucose clearance from the circulating perfusion media was also assessed separately during precontraction and postcontraction, and glucose transport was determined in muscle taken after 25 min of recovery using ¹⁴C 2-deoxyglucose (¹⁴C 2DOG).