Fig. 1.

Postweaning responses of male Fischer 344 rats to dietary methionine restriction (MR) or 40% dietary restriction (DR). At 32 days of age, rats were randomly assigned to groups that would receive a control diet containing 0.86% methionine (control group), a diet with methionine restricted to 0.17% (MR), or a DR group that would receive the control diet at 60% of the consumption of the control group. Energy intake (A), body weight (B), relative adiposity (white adipose tissue, WAT) (C), and brown adipose tissue (BAT) UCP1 mRNA (F) were measured in separate cohorts of 8 rats per treatment per time point after 2, 4, and 8 wk on the respective diets. Plasma leptin and adiponectin (D) were measured in samples from the 8-wk time point, as were the mRNA levels for leptin, adiponectin, and UCP1 in the WAT and BAT tissues collected after 8 wk (E). EWAT, epididymal white adipose tissue; IWAT, inguinal white adipose tissue; RPWAT, retroperitoneal white adipose tissue. Response variables were analyzed by ANOVA. ^a,b,cMeans at each time point with letters that differ denote P < 0.05. E: *P < 0.05, significant difference for each gene and tissue compared with control group.