TABLE 1.
Primer name | Sequence (5′ to 3′)a |
---|---|
Domain 1 for | CGCGGATCCAGAAGTTAAACAGGAG |
Domain 1 rev | AAAACTGCAGTCAATGATGATGATGATGATGAGCTGCCACAAG |
Domain 2 for | CGGGATCCAGCAGCTTATCCGATTGTACATGTAGAT |
Domain 2 rev | CCCAAGCTTTCAATGATGATGATGATGATGTGTTTCTTGAATTTG |
Domain 3 for | GGATCCACTGCACGTATCATTTTTAATGGTAA |
Domain 3 rev | CCCAAGCTTTCAATGATGATGATGATGATGACGTTTATCGCGAATCAGAATATT |
Domain 4 for | CGCGGATCCATTTCATTATGATAGAAATAACATAGCAGTTGGG |
Domain 4 rev | CCCAAGCTTTCAATGATGATGATGATGATGTCCTATCTCATAGCC |
Restriction sites are underlined.