TABLE 1.

Sequences and annealing temperatures for real-time PCR primers

Cytokine	Forward primer (5′-3′)	Reverse primer (5′-3′)	Annealing temp (°C)
IL-1β	GTGAGGCTCAACATTGCGCTGTA	TGTCCAGGCGGTAGAAGATGAAG	64
IL-10	AGCAGATCAAGGAGACGTTC	ATCAGCAGGTACTCCTCGAT	55
IL-12p40	TTGCCGAAGAGCACCAGCCG	CGGTGTGCTCCAGGTCTTGGG	64
IL-18	GAAACGTCAATAGCCAGTTGC	TCCCATGCTCTTTCTCACAACA	64
IFN-γ	ACACTGACAAGTCAAAGCCGC	AGTCGTTCATCGGGAGCTTG	60
TGF-β4	CGGCCGACGATGAGTGGCTC	CGGGGCCCATCTCACAGGGA	60
β-Actin	CAACACAGTGCTGTCTGGTGGTA	ATCGTACTCCTGCTTGCTGATCC	58