FIG. 3.

CCR5 expression on macrophages and spleen cells after incubation with TgCyp18. (A) Peritoneal macrophages (1 × 10⁶) from B6 mice were incubated with 50 μg/ml TgCyp18, MTgCyp18, or GST or 10 ng/ml LPS for 24 h. (B) Spleen cells (1 × 10⁶) from B6 mice incubated with 50 μg/ml TgCyp18, MTgCyp18, or GST or 5 μg/ml ConA for 24 h; the cells were subjected to flow cytometry. Shaded profiles correspond to cells labeled with isotype controls. Numbers correspond to the percentage of labeled cells. Data are representative of two separate experiments with similar results.