TABLE 1.
Results of in vitro peptide binding assay for HLA-A11-restricted cytotoxic-T-lymphocyte epitopes derived from HPV-58 E6, E7, and L1 proteins
| Protein | Peptide sequence | Amino acid position | Relative binding ability (%) |
|---|---|---|---|
| HPV-58 L1 | SIKSPNNNK | 77-85 | 249.9 |
| RVRLPDPNK | 101-109 | 387.3 | |
| AVPDDLYIK | 296-304 | 406.2 | |
| TSESQLFNK | 327-335 | 126.9 | |
| MTLCTEVTK | 368-376 | 332.3 | |
| EVTKEGTYK | 373-381 | 23.2 | |
| GTYKNDNFK | 378-386 | 321.6 | |
| LQFVFQLCK | 397-405 | 255.7 | |
| YTFWEVNLK | 469-477 | 181.9 | |
| LLQSGLKAK | 494-502 | 7.0 | |
| PTTRAPSTK | 510-518 | 48.6 | |
| RAPSTKRKK | 513-521 | 9.8 | |
| STKRKKVKK | 516-524 | 145.6 | |
| HPV-58 E6 | QALETSVHE | 17-25 | 0 |
| TSVHEIELK | 21-29 | 58.7 | |
| KVCLRLLSK | 64-72 | 142.2 | |
| SLYGDTLEQ | 82-90 | 0 | |
| DTLEQTLKK | 86-94 | 7.6 | |
| KCLNEILIR | 94-102 | 56.4 | |
| RPLCPQEKK | 108-116 | 3.2 | |
| HPV-58 E7 | CYTCGTTVR | 59-67 | 10.4 |
| LCINSTATE | 68-76 | 0 | |
| ATEVRTLQQ | 74-82 | 0 | |
| CTIVCPSCA | 88-96 | 0.8 | |
| TIVCPSCAQ | 89-97 | 3.3 | |
| EBV EBNA3B (positive control) | IVTDFSVIK | 416-424 | 100 |