TABLE 6.
IFN-γ ELISPOT results according to HLA type and cervical status
| HLA | Encoding region, amino acid positiona | Cervical status | No. with positive ELISPOT result/no. tested (%)b | Mean IFN-γ spot-forming count (range, SD)/104 cells | P valuec |
|---|---|---|---|---|---|
| A11 | L1, 101-109 | Cleared infection | 7/17 (41.2) | 31.7 (18-43, 9.4) | <0.001 |
| CIN2 | 0/10 (0) | ||||
| CIN3 | 0/10 (0) | ||||
| ICC | 0/16 (0) | ||||
| A11 | L1, 296-304 | Cleared infection | 2/17 (11.8) | 32.5 (29-36, 4.9) | 1.0 |
| CIN2 | 4/10 (40.0) | 16.3 (12-21, 4.0) | |||
| CIN3 | 0/10 (0) | ||||
| ICC | 0/16 (0) | ||||
| A11 | L1, 327-335 | Cleared infection | 6/17 (35.3) | 35.5 (26-54, 10.4) | <0.001 |
| CIN2 | 0/10 (0) | ||||
| CIN3 | 0/10 (0) | ||||
| ICC | 0/16 (0) | ||||
| A11 | L1, 469-477 | Cleared infection | 8/17 (47.1) | 33.0 (16-45, 10.2) | <0.001 |
| CIN2 | 0/10 (0) | ||||
| CIN3 | 0/10 (0) | ||||
| ICC | 0/16 (0) | ||||
| A11 | E6, 64-72 | Cleared infection | 1/17 (5.9) | 15.0 | 0.408 |
| CIN2 | 2/10 (20.0) | 16.0 (15-17, 1.4) | |||
| CIN3 | 0/10 (0) | ||||
| ICC | 4/16 (25.0) | 19.3 (14-25, 4.8) | |||
| A11 | E6, 94-102 | Cleared infection | 0/17 (0) | 1.0 | |
| CIN2 | 2/10 (20.0) | 14.7 (11-18, 3.5) | |||
| CIN3 | 0/10 (0) | ||||
| ICC | 0/16 (0) | ||||
| A11 | E7, 74-82 | Cleared infection | 3/17 (17.6) | 24.0 (18-30, 6.1) | 0.730 |
| CIN2 | 4/10 (40.0) | 19.8 (12-28, 7.5) | |||
| CIN3 | 2/10 (20.0) | 14.0 (12-16, 2.8) | |||
| ICC | 3/16 (18.8) | 16.0 (13-20, 3.6) | |||
| A11 | E7, 78-86 | Cleared infection | 0/17 (0) | 1.0 | |
| CIN2 | 2/10 (20.0) | 13.0 (12-14, 1.4) | |||
| CIN3 | 0/10 (0) | ||||
| ICC | 0/16 (0) | ||||
| A11 | E7, 88-96 | Cleared infection | 0/17 (0) | 1.0 | |
| CIN2 | 0/10 (0) | ||||
| CIN3 | 0/10 (0) | ||||
| ICC | 2/16 (12.5) | 13.0 (15-20, 3.5) | |||
| A2 | E6, 99-107 | Cleared infection | 0/15 (0) | 1.0 | |
| CIN2 | 2/12 (16.7) | 14.0 (11-17, 4.2) | |||
| CIN3 | 0/5 (0) | ||||
| ICC | 0/16 (0) | ||||
| A24 | L1, 468-476 | Cleared infection | 0/14 (0) | 1.0 | |
| CIN2 | 2/4 (50.0) | 15.0 (12-18, 4.3) | |||
| CIN3 | 0/9 (0) | ||||
| ICC | 0/12 (0) |
a
Amino acid position numbering is according to the HPV-58 prototype (GenBank accession no. D90400).
b
Subjects with heterozygous HLA-A alleles were tested for each of the HLA types and, therefore, counted twice.
c
Positive response rate among subjects with cleared infection compared to that in subjects with CIN2/CIN3/ICC by 2-tailed Fisher's exact test.