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Oligonucleotide sequences designed for PCR
| Primer | Oligonucleotide sequence (5′-3′) | Product size (bp) |
|---|---|---|
| PNF (forward) | 5′-AAAACCAGTCCAGAGGCAAG-3′ | 250a |
| PNR (reverse) | 5′-CGGATCAGACGCACAGTATG-3′ | |
| PNPF (forward) | 5′-CCCTAGTGAGCGGCAATTGT-3′ | 60b |
| PNPR (reverse) | 5′-TCCAGCGCCCTGATTGAA-3′ |
PNF and PNR were designed for the amplification of a conserved region of PRRSV ORF7 sequence (250 bp).
Designed for another region of the amplification of a conserved region of PRRSV ORF7 sequence (250 bp).
