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. 2010 Jul 14;17(9):1428–1438. doi: 10.1128/CVI.00195-10

TABLE 1.

Primer sequences for proteases and apolipoprotein

Protein Name Primer sequences (5′-3′)a Primer restriction sites Size (aa) Molecular mass (kDa) GenBank accession no. Reference(s)
Active cysteine protease Yv5032C08 aactgcagAAATTGGGCGAGCTCGATCTG, cccaagcttTCAAGCTACAGTTTCTTTGCCCAG PstI, HindIII 227 25 AY525149 27
Inactive cysteine protease Yv5009F04 cgggatccAAACTACCAAAATGGTTCGATC, ttctgcagTCATTCAAAATCTTCAGGCTC BamHI, PstI 240 28 AY525155 27
Active serine protease Yv7016G03 accggtcgacATTGTCGGCGGTCGTTTAGCTAAGCC, accgctgcagTTAATTATTTCTAAGGATATTTTTGATCCATTGG SalI, PstI 231 26 AY333071 7
Apolipoprotein Ssag1.2 ggggatccTCGAATGTGAAACGAAACAATG, ggctgcagnnCTGCAAATATTGTCTGATAGC BamHI, PstI 393 46 AF462196 25, 21
a

S. scabiei-specific sequence shown in uppercase.