TABLE 1.

Cross-reactivity of MAbs raised against anthrose-containing synthetic carbohydrates with spores of Bacillus spp.^a

Bacillus sp.	Strain	Code	Plasmid content	OD from spore ELISA of:		Mean fluorescence intensity (Luminex assay)
				MTA1	MTD6
B. anthracis	Ames	Ba0	pX01+ pX02+	0.44	1.41	19,398
	Böhm A58	Ba1	pX01− pX02−	0.33	1.54	18,719
	Böhm A1	Ba2	pX01+ pX02−	1.01	1.66	7,229
	Böhm A73202.2000	Ba3	pX01− pX02+	1.31	2.16	21,591
	NCTC 8234 Sterne	Ba4	pX01+ pX02−	1.76	2.36	11,120
	NCTC 02620	Ba7	pX01+ pX02+	0.23	0.77	24,262
B. cereus	ATCC 10876	Bc1		1.52	2.09	23,710
	ATCC 13061	Bc2		−0.09	−0.05	141
	ATCC 14579	Bc3		−0.04	−0.05	300
	ATCC 33019	Bc4		0.24	1.15	5,964
	ATCC 11778	Bc5		0.01	0.07	470
B. subtilis	ATCC 6051	Bs1		−0.10	−0.13	28
	ATCC 6633	Bs2		−0.10	−0.14	26
	ATCC 11774	Bs3		−0.11	−0.13	23
	Biocontrol AG	Bs4		ND	ND	38
B. thuringiensis	Kurstaki SP09	Bt1		−0.02	−0.03	47
	ATCC 10792	Bt2		−0.09	−0.13	33
B. atrophaeus	ATCC 9372	Bat		−0.10	−0.14	27
B. circulans	ATCC 61	Bcir		ND	ND	33
B. licheniformis	ATCC 12759	Bl1		−0.10	−0.11	73
	ATCC 14580	Bl2		−0.08	−0.12	27
B. megaterium	ATCC 9885	Bm1		−0.10	−0.13	23
	ATCC 14581	Bm2		−0.10	−0.13	28
B. sphaericus	ATCC 4525	Bsp		−0.08	−0.13	23
B. pumilus	ATCC 14884	Bp		−0.10	−0.13	28

^aB. anthracis strains were chemically inactivated by paraformaldehyde with the exception of the Ames strain (Ba0), which was gamma irradiated. All the other Bacillus species and strains were not inactivated. Spores were used at a concentration of 1 × 10⁶/ml. ELISA results are expressed as test optical densities minus two times the blank (blank, 0.10). Luminex values are shown as the mean fluorescence intensities from two experiments. Bolded values indicate MAb cross-reactive samples. ND, not done.