TABLE 4.

Comparison of f-AbBA detection limits in H3, H1, and H5 HAs with corresponding HI titers using equivalent viruses

Antigen	Ferret antiserum to influenza virus	f-AbBA dilutiona	Virus/HI titerb
H3
H3-BR07	H3-BR07	1,280	1,280
	H3-WI05-L	2,560	2,560
	H3-CA04	320	320
	H3-Syd97	<80	<10
H3-WI05-L	H3-BR07	320	320
	H3-WI05-L	2,560	2,560
	H3-CA04	640	320
	H3-Syd97	<80	<10
H3-CA04	H3-BR07	160	640
	H3-WI05-L	1,280	2,560
	H3-CA04	2,560	5,120
	H3-Syd97	<80	<10
H1
H1-BR07	H1-BR07	1,280	640
	H1-SI06	640	160
	H1-NC99	320	<10
	H5-VN04	80	NDc
H1-SI06	H1-BR07	1,280	320
	H1-SI06	640	640
	H1-NC99	640	<10
	H5-VN04	<80	ND
H1-NC99	H1-BR07	320	160
	H1-SI06	320	160-320
	H1-NC99	1,280	2,560
	H5-VN04	<80	ND
H5
H5-VN04	VN04 clade 1	640	160
	INDO05 clade 2.1	640	80
	BHG05 clade 2.2	160	<10
	EG06 clade 2.2	80	80
	ANH05 clade 2.3.4	2,560	160-320

^aMaximum dilution that gave >10% binding signal compared to homologous serum binding at 1:80 dilution.

^bVirus titer determined by HI assay.

^cND, not determined.