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Dose escalation of Ad5.HA and induction of antibodies. Five groups of nine 5-week-old chickens were subcutaneously immunized with indicated doses of Ad5.HA (1010, 109, 108, 107, 106, 105, or 104 vp/chicken) or Ad5 (1010 vp). Sera from chickens were isolated at indicated time points and tested in an HI assay using 8 HA units and turkey red blood cells. Results are shown as the log2 value of the geometric mean (GMT) of each group.
