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. 2010 Jul 2;299(3):H799–H810. doi: 10.1152/ajpheart.00170.2010

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Fig. 1. — Reduction of high-affinity choline uptake (HACU) and choline transporter (CHT) expression in CHT heterozygous (Het; CHT^+/−) atrial preparations. A: in cardiac atrial preparations, CHT^+/− tissue showed diminished hemicholinium-3 (HC-3)-sensitive [³H]choline uptake compared with wild-type (WT; CHT^+/+) atria. CPM, counts/min. B: CHT expression was significantly reduced in CHT^+/− atria compared with CHT^+/+ atria. C and D: quantitation of CHT and M₂ muscarinic ACh receptor (M₂AChR) expression in CHT^+/+ and CHT^+/− mice. The mouse monoclonal CHT antibody recognizes the same mature glycosylated and immature protein. Values are means ± SE; n = 4 mice/genotype. Significance was determined using a one-tailed, unpaired Student's t-test (*P < 0.05).