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Cardiac tissue levels of ACh levels are lower in CHT+/− mice. A: tissue ACh levels measured by HPLC were significantly lower in whole hearts of CHT+/− mice. B: tissue levels of choline were lower in CHT+/− versus CHT+/+ mice. Values are means ± SE; n = 9 mice/genotype. Significance was determined using a one-tailed, unpaired Student's t-test (*P < 0.05).
