Fig. 7.

The effect of sildenafil on Na⁺/K⁺-ATPase activity. Isolated mouse hearts were stabilized and aerobically perfused with KBH for 40 min. Mouse hearts were then subjected to aerobic perfusion with Rb-KBH for 10 min, or 30 min global ischemia followed by 10 min reperfusion with Rb-KBH in the absence or presence of 0.1 μM sildenafil. Clearly, ischemia and reperfusion lead to Na⁺/K⁺-ATPase inhibition, and this is partially alleviated by sildenafil treatment. Bars represent means ± SE; n = 5 to 6 animals. *P < 0.05 vs. control ischemia-reperfusion (1-way ANOVA).