Fig. 2.

DM/HC pigs exhibit aberrant Akt signaling including hypoactivation of Akt and activation of GSK-3β. A: Western blot analyses of whole coronary artery lysates from animals randomized into 4 experimental groups for 24 wk with antibodies against phosphorylated (p)-Akt (Ser473), Akt, p-GSK-3β (Ser9), and GSK-3β. B: densitometry results are expressed as a fold change compared with the control group, with β-actin as the internal loading control. *P < 0.01 for p-Akt, P < 0.05 for p-GSK-3β for DM/HC group.