Fig. 1.

Altered intracellular localization of Tec tyrosine kinase in adult cardiac myocytes. A: adult mouse myocytes were isolated by retrograde perfusion with collagenase and examined immediately (fresh isolation) or after 24 h in culture. Cells were fixed, and Tec was visualized by immunodecoration, demonstrating a striated localization pattern that returned to a dispersed, cytoplasmic arrangement after culture. B: colabeling with caveolin (Cav)-3 demonstrated the localization of Tec to T-tubules, a pattern that reverted to the diffuse cytoplasmic distribution after culture. Bar = 10 μM.