Fig. 4.

Tec exhibits a striated localization pattern after adenine nucleotide stimulation. Adult mouse myocytes were isolated and cultured for 24 h, after which they were subjected to hypoxia alone (95% N₂-5% CO₂ for 1.5 h), hypoxia plus 1.5 h of reoxygenation, or ATP/adenosine stimulation. Tec localization was then evaluated by immunolabeling and confocal microscopy. A: hypoxia alone or with reoxygenation produced no discernable alterations in Tec localization. B: ATP stimulation induced Tec translocation to structures resembling T-tubular striation (left; adenosine treatment produced similar effects, not shown). PBS vehicle was used as a control. Inhibition of Tec with terreic acid (TA) blocked this translocation (right panels). Bar = 5 μm. Please see Supplemental Fig. 5 for lower-magnification images.