TABLE 2.
Luminescence analysis of mutants involved in the AinS signaling pathways
| Strain | Reference | Genotype | Patches on LBS platesa |
|---|---|---|---|
| ES114 | 8 | Wild type | − |
| NL2 | This study | ES114 ainS::mini-Tn5-ermR | + |
| NL43 | This study | ES114 ΔainR | − |
| NL55 | This study | ES114 ΔainSR | + |
| CL24 | 47 | ES114 luxS::kanR | − |
| NL11 | This study | ES114 ainS::mini-Tn5-ermR litR::kanR | − |
| CL39 | 46 | ES114 luxI-ainS::catR | − |
| CL41 | 46 | ES114 ainS::catR luxS::kanR | − |
| CL64 | 47 | ES114 ainS::catR luxO::kanR | + |
| CL90 | 46 | ES114 luxS::kanR luxO::kanR | − |
| CL91 | 46 | ES114 ainS::catR luxS::kanR luxO::kanR | + |
a
All strains were patched onto LBS plates, and the results of the negative images are reported as follows: +, patches were bright; −, patches were not bright (compared to ainS mutant patches [Table 1]).