TABLE 3.

Functional classification of ICEEc2 genes encoding proteins with putative functions

Cellular process	Gene	Putative product	Reference or source
DNA processing enzymes
DNA replication	ORF87	Single-stranded binding protein	Swiss-Prot P25762
	ORF90	DNA topoisomerase IA	Swiss-Prot P14294
	ORF111	CbiA-like chromosome partitioning protein	TrEMBL A7ZRE7
DNA recombination	ORF1	XerC-like recombinase	Swiss-Prot Q9F771
	ORF18	Rci recombinase	Swiss-Prot P16470
DNA transposition	ORF33-ORF37 (tnsABCDE)	Tn7 transposition proteins	Swiss-Prot P13988, P13989, P05846, P13991, P05845
	ORF86	YhgA-like transposase	Swiss-Prot P31667
DNA unwinding	ORF2	Putative helicase	TrEMBL A7ZRD0
	ORF3	UvrD-like helicase	Swiss-Prot Q8K2I9
	ORF109	DnaB-type helicase	Swiss-Prot P0ACB0
DNA nuclease	ORF108	ParB nuclease	Swiss-Prot Q92JI0
DNA cleavage	ORF79	Type IV restriction enzyme	Swiss-Prot P24202
Conjugative transfer
Formation of type IV pilus	ORF6-ORF17 (pil genes)	Type IV pilus proteins	28
Coupling	ORF76	TraD-like coupling protein	TrEMBL C2LLJ7
Mating pair stabilization	ORF58	TraG-like protein	Swiss-Prot P33790
Other	ORF65	TraC-like protein	TrEMBL C2LLI5
	ORF67	TrbI protein	Swiss-Prot P05359
Xenobiotic resistance/adaptation to the environment
Antibiotic resistance	ORF26 (dfrA1)	Trimethoprim resistance	Swiss-Prot P00382
	ORF27 (sat2)	Streptothricin resistance	Swiss-Prot P13018
	ORF28 (aadA1)	Streptomycin/spectinomycin resistance	Swiss-Prot P0AG06
Iron acquisition	ORF63 (iha)	Catecholate receptor/potential adhesin	TrEMBL Q6EZC5
Miscellaneous	ORF45	Antirestriction protein	TrEMBL B4TL18
	ORF55	L31 ribosomal protein	Swiss-Prot B7L427
	ORF94	Sb34 phage protein	TrEMBL Q8HAA4
	ORF101	Ea22 phage protein	Swiss-Prot P03756
	ORF103	Ea22 phage protein	Swiss-Prot P03756