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. 2010 Jul 16;192(19):4876–4884. doi: 10.1128/JB.00401-10

FIG. 3.

FIG. 3.

Zymogram analysis of nuclease activity of MG_186. Whole-cell lysates of M. genitalium were prepared by sonication, and samples were run on 10% SDS-PAGE gels impregnated with herring sperm DNA. Nuclease activity was detected as detailed in Materials and Methods. Purified rMG_186 was also analyzed for nuclease activity by zymogram analysis. Lane 1, Coomassie blue staining of whole-cell lysates of M. genitalium; lane 2, zymogram analysis of lane 1 before Coomassie blue staining; and lane 3, zymogram analysis of purified rMG_186.