FIG. 5.
Substrate specificity of OdhA muteins. The left 6-column set shows succinyl-CoA utilization, and the right 6-column set CoA liberation, where each column indicates the amount of substrate consumed and product formed, respectively, after 3, 6, 9, 12, and 15 min (from left to right). The assays included 50 μg OdhA protein either from unmutated OdhA (WT), OdhA protein with the point mutation(s) as indicated, or no protein (− enzyme). The bars represent the areas (10−3), and the initial concentration for succinyl-CoA added was 150 μM.