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Effect of DTT on stimulating NrdH-dependent S. aureus NrdEF ribonucleotide reductase activity. NrdE (20 μg) and NrdF (10 μg) were incubated in the standard assay (see Materials and Methods) in the presence of 4.5 μg of the NrdH protein (▪) or in the absence of the NrdH protein (○).
