FIG. 4.

KatE is the predominant detoxifier of 5 μM exogenous H₂O₂ in B. abortus 2308. Shown are the levels of peroxides measured using the xylenol orange/ferrous iron assay in B. abortus 2308 (•), KH16 (2308 ahpCD) (▪), KH2 (2308 katE) (▴), and KK21 (2308 ahpCD katE) (▾) cell suspensions at selected times after the addition of 5 μM H₂O₂. The data presented here are representative of multiple (≥3) experiments performed from which equivalent results were obtained. “EP” denotes the levels of endogenous peroxides detected prior to the addition of the H₂O₂; “↓” denotes the levels of endogenous intracellular peroxides detected immediately after the addition of the H₂O₂.