FIG. 6.

The B. abortus ahpCD mutant KH16 exhibits an increased sensitivity to the peroxynitrite generator SIN-1. Viability of B. abortus 2308 (white bars), KH16 (2308 ahpCD) (black bars), and KH16 [pMWV77] (gray bars) before and after a 60-min exposure to SIN-1. The data presented are means and standard deviations for triplicate determinations for each strain in a single experiment. The data presented here are representative of multiple (≥6) experiments performed from which equivalent results and statistical trends were obtained. Statistical significance (P ≤ 0.05) as determined by the Student two-tailed t test for comparison of 2308 versus the other strains is represented by an asterisk (*).