FIG. 3.
Growth characteristics of T. kodakarensis lacking TK0240, TK0474, and/or TK0882 genes. (A and B) Growth curves of T. kodakarensis KU216 (ΔpyrF), DUH2 (ΔpyrF ΔTK0240), DUH4 (ΔpyrF ΔTK0474), and DUH8 (ΔpyrF ΔTK0882) grown at 85°C (A) and 93°C (B). (C and D) Growth curves of the disruptant strains DUH24 (ΔpyrF ΔTK0240 ΔTK0474), DUH28 (ΔpyrF ΔTK0240 ΔTK0882), DUH48 (ΔpyrF ΔTK0474 ΔTK0882), and DUH248 (ΔpyrF ΔTK0240 ΔTK0474 ΔTK0882) and original strain KU216 (ΔpyrF) grown at 85°C (C) and 93°C (D). OD660, optical density at 660 nm.