TABLE 3.

Significance of the subcellular localization of immunogold labeling for putative inclusion membrane proteins based on stereological analysis

Gene ID	Protein	Association of the immunogold particles witha:
		P. amoebophila		Inclusion		Amoeba cytoplasm	Mitochondria
		RLI	P value	RLI	P value	RLI	RLI
pc0156	IncQ	7.6	<0.0005	2.37	<0.0005	0.28	0.3
pc0399	IncA	1.79	<0.0005	6.32	<0.0005	0.29	0.6
pc0530	IncR	4.0	<0.0005	1.2	<0.0025	0.7	0.6
pc0577		2.51	<0.01	1.3	NS	0.99	0.45
pc1111	IncS	2.76	<0.0005	1.72	<0.0005	0.77	0.31

^aP values are based on a chi-square test; statistical tests were not performed if the relative labeling index (RLI) was <1. NS, not significant.