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. 2010 Jul 30;192(19):4868–4875. doi: 10.1128/JB.00550-10

FIG. 4.

FIG. 4.

Schematic diagram depicting the chimeric DosST and DosTS HKs (A) and complementation analysis using the ΔdevS mutant strain of M. smegmatis with DosST and DosTS (B). The chimeric DosST HK consists of the GAF-A domain of DosS (M1 to A218) and the GAF-B and kinase domains of DosT (T217 to R573). The chimeric DosTS HK consists of the GAF-A domain of DosT (M1 to A216) and the GAF-B and kinase domains of DosS (T219 to Q578). The plasmids pNBV1DosST and pNBV1DosTS, containing the genes encoding DosST and DosTS, respectively, were introduced into the ΔdevS mutant strain of M. smegmatis, and the complementation test was performed by determining the expression levels of hspX in M. smegmatis strains grown under either aerobic (+O2) or hypoxic (−O2) conditions for 20 and 50 h by means of RT-PCR. RT-PCR for the 16S rRNA gene was performed to ensure that the same amounts of total RNA were employed in RT-PCR.