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. 2010 Jul 30;192(19):4868–4875. doi: 10.1128/JB.00550-10

FIG. 6.

FIG. 6.

Effect of E87G, H89R, D90G, H97E, V108R, L118R, and T169N mutations on the sensory function of DosS in vivo. Complementation tests were performed by determining the expression levels of hspX in M. smegmatis strains grown under either aerobic (O2+) or hypoxic (O2−) conditions for 20 h by means of RT-PCR (A) and qRT-PCR (B). The ΔdevS mutant strains were complemented with the wild-type dosS and dosT as well as the E87G, H89R, D90G, H97E, V108R, L118R, and T169N mutant forms of dosS. As a control, the ΔdevS mutant strain of M. smegmatis containing the empty vector pNBV1 was included in the experiment. The strains were grown under either aerobic (O2+) or hypoxic (O2−) conditions for 20 h. The levels of mRNA specific for hspX were determined by qRT-PCR and normalized to those of 16S rRNA. Fold induction of hspX expression indicates the level of hspX mRNA of hypoxic culture relative to that of aerobic culture. All values provided are the averages of results of two independent determinations. Error bars indicate standard deviations.