TABLE 2.
Primers used in this study
| Primer function and name | Nucleotide sequence (5′-3′)a | Source or reference |
|---|---|---|
| Construction of plasmids for allelic replacement | ||
| agr.upF | ATTTA GAATTC ATATGAATGCTGAAGTAGAT | This study |
| agr.upR | ATTAA GGATCC GTTATCTTCGTATAGTACTA | This study |
| agr.downF | ATTTA CTGCAG TTCAATTGTAAATCTTGTTG | This study |
| agr.downR | AATAT AAGCTT TTGATACATCTAGTATAGAA | This study |
| sarA.upF | ATTTA GAATTC GAGACTTTATTCATATGCTT | This study |
| sarA.upR | ATTTA GGATCC CTTAAGAATGAGTTGACTAT | This study |
| sarA.downF | ATTTA CTGCAG TGATAGATGATACATTCTAT | This study |
| sarA.downR | ATTAT AAGCTT TTATCTTCACGTACAAGATT | This study |
| Primer extension | ||
| spa.PEbio1 | BIO-TGTTACGCCACCAGATATAA | This study |
| spa.PEbio3 | BIO-ACCTAGTTTACGAATTGAATA | This study |
| Amplification of DIG-labeled probes | ||
| SA1665F | TTCGTATAGAGGCTGGTTAG | 23 |
| SA1665R | AATTGGTTGGTTATCTGGAT | 23 |
| spaF | TGTAGGTATTGCATCTGTAA | 52 |
| spaR | AAGTTAGGCATATTCAAGAT | 52 |
| sarSF | TTGATGAGCGTAATACTTAC | 52 |
| sarSR | GAGCTAATAATTGTTCAGCA | 52 |
| RNAIII.F | TATAACTAGATCACAGAGATG | This study |
| RNAIII.R | TTAGTACTATACGAAGATAAC | This study |
| sarA.F3 | TATTGACATACATCAGCGAA | This study |
| sarA.R1 | GTTTGCTTCAGTGATTCGTT | This study |
| fadXF | TAGCGAATGATATTCATAGT | This study |
| fadXR | TAGTTGATAGTCGTCAACTA | This study |
| SAR0995F | ATCATTGAACGCTCTTGCAA | This study |
| SAR0995R | ATAGCGTGTCAATGCTTCAA | This study |
| SAR0996F | CTAATGTTAAACATACAACT | This study |
| SAR0996R | TATTCTGCCGTATATTGATT | This study |
| SAR1741F | GTATGAATTAATGCCGATTA | This study |
| SAR1741R | CGTCACCATAACCAATATAT | This study |
| SAR2413F | CAACGCTTACAAGGCTATAA | This study |
| SAR2413R | GCTACACAGTTCACTCTAAT | This study |
| SAR2589F | GGTTATGCATTAATGCAAGT | This study |
| SAR2589R | TAACGTGGAATACTTCTGTT | This study |
| mtlAF | CCAATGTTACTTGGTGCAAT | This study |
| mtlAR | TAAGTTGCAACACCAGTCAT | This study |
| sarT-U.F | GATTGGTCTATACCGATATA | This study |
| sarT-U.R | GTTGTATTGGAGTAACTGAA | This study |
| Amplification of spa promoter fragments for lacZ reporter gene constructs | ||
| spa.lacZF1 | AATTT GGATCC GAATCAATTATTAGCAGATAA | This study |
| spa.lacZF2 | AATTT GGATCC TCAGCACATAATGAACAACTT | This study |
| spa.lacZF3 | AATTT GGATCC TGTATTAAACCGCTTTCATTA | This study |
| spa.lacZF4 | AATTT GGATCC ACTTCCTGAATAAATCTTTCA | This study |
| spa.lacZF5 | AATTT GGATCC AAATATCTCTATATTTTATC | This study |
| spa.lacZF6 | AATTT GGATCC TATTAATCGAAATAGCGTGA | This study |
| spa.lacZF7 | AATTT GGATCC TTATAAGTTGTAAAACTTAC | This study |
| spa.lacZF8 | AATTT GGATCC AATATAGATTTTAGTATTGC | This study |
| spa.lacZF9 | AATTT GGATCC GTATTGCAATACATAATTCG | This study |
| spa.lacZR | AATTT GAATTC ACCTAGTTTACGAATTGAATA | This study |
a
Restriction sites are underlined; BIO denotes 5′ biotinylation.