TABLE 2.
Acetylene reduction of peas infected with various strains of R. leguminosaruma
| Strain (genotype) | ARA | No. of harvests | Total no. of plants | Location | P value |
|---|---|---|---|---|---|
| Rlv3841 (wild type) | 3.88 ± 0.18 | 5 | 40 | RDG | |
| RU3941 (dmeΩ) | 5.78 ± 0.25 | 5 | 40 | RDG | <0.001 |
| RU4008 (tmeΩ) | 3.52 ± 0.17 | 2 | 10 | RDG | 0.395 |
| RU4009 (dmeΩ tmeΩ) | 5.56 ± 0.40 | 2 | 10 | RDG | <0.001 |
| RU4294 (pykA D220dme tmeΩ) | 0.28 ± 0.17 | 1 | 5 | RDG | <0.001 |
| RU4336 (pckA D220dme tmeΩ) | 0.01 ± 0.00 | 1 | 5 | RDG | <0.001 |
| RU4039 (pckA) | 5.06 ± 0.69 | 2 | 15 | RDG | 0.061 |
| RU4291 (pykA) | 6.75 ± 0.61 | 1 | 5 | RDG | <0.001 |
| RU4166 (D220dme) | 2.24 ± 0.43 | 1 | 10 | RDG | 0.056 |
| RU4335 (pckA D220dme) | 0.02 ± 0.01 | 1 | 5 | RDG | <0.001 |
| Rlv3841 (wild type) | 2.61 ± 0.20 | 3 | 18 | JIC | |
| RU3941 (dmeΩ) | 3.77 ± 0.52 | 2 | 11 | JIC | 0.005 |
| RU4166 (D220dme) | 1.91 ± 0.12 | 1 | 4 | JIC | 0.010 |
| RU4167 (D220dme tmeΩ) | 1.24 ± 0.16 | 1 | 7 | JIC | 0.011 |
| LMB139 (Δdme) | 1.65 ± 0.13 | 3 | 20 | JIC | <0.001 |
| LMB141 (Δpta) | 3.84 ± 0.27 | 2 | 14 | JIC | <0.001 |
| LMB194 (Δme) | 1.64 ± 0.20 | 1 | 8 | JIC | 0.029 |
| RU4336/pdme | 2.06 ± 0.14 | 1 | 7 | JIC | 0.962 |
| RU4336/ppta | 0 | 1 | 7 | JIC | <0.001 |
| RU4336/pme | 2.14 ± 0.21 | 1 | 7 | JIC | 0.828 |
Strains are shown with their genotype in parentheses. The number of independent plant harvests and total number of plants are shown for each strain. Harvests were conducted at two locations, the University of Reading (RDG) and the John Innes Centre (JIC). All samples were compared by one-way blocked analysis of variance (ANOVA) for plants infected with Rlv3841 from the same harvests. Strain RU3941, strain RU4166, and the wild type (3841) were harvested at both RDG and JIC. The P value from blocked ANOVA across both sites for RU3941 versus 3841 is <0.001, and that for RU4166 versus 3841 is 0.006. Rates of acetylene reduction (ARA) are in micromoles h−1 plant−1 and are shown ± standard errors of the means (SEM). Plasmids pLMB127 (pdme), pLMB128 (ppta), and pLMB129 (pme) contain the whole dme gene, just the Pta domain, and just the malic enzyme domain, respectively.