FIG. 2.

A 13-mer phosphorothioate 2′ deoxyribose oligomer does not cause physical disruption of intact human vaginal or foreskin tissue at the histological level. Histological H&E staining shows that treatment with 25 μM OPB-T did not disrupt squamous epithelium in human vaginal (A, B) or foreskin (C, D) tissue explants compared to the level for the untreated controls. The untreated controls (A, C) show tissue integrity, and the 25 μM OPB-T-treated tissues (B, D) do not exhibit epithelial disruption from a histological perspective. Tissues were treated or untreated with OPB-T for 24 h. Arrows indicate the basal layer. Above the basal layer is the squamous epithelium, and below is the stroma, the connective tissue. All magnifications shown are ×20. Representative data from 2 vaginal and 2 foreskin tissues tested are shown.