FIG. 2.

Effect of time of drug addition on the inhibition of HIV entry inhibitors. MT-4 cells infected with HIV-1 NL4-3 at a multiplicity of infection of 0.5 at 20°C for 1 h and 37°C thereafter were treated during virus infection and/or at various times postinfection. Treatment with test compounds alone (A) or in combination with BMS-155 (B) or ADS-J1 (C) was performed at replication-blocking concentrations (roughly 100-fold the 50% effective concentration [EC₅₀]). Viral p24 levels in the culture medium were monitored at 30 h postinfection. The figure shows a representative result of three experiments.